Keywords
malondialdehyde
lipid oxidation
performances parameters
thiobarbituric acid reactive substances
How to Cite
Abstract
A method has been described and verified to determine the oxidation index in meat, through the quantification of thiobarbituric acid reactive substances (TBARS). The performance parameters evaluated were the verification of the wavelength at which the maximum absorbance occurs; working range of the instrument, limit of detection of the instrument (LDI); limit of detection and quantification of the method (LDM and LQM); method working range and precision such as repeatability and intermediate precision. It was found that the maximum absorbance occurs at 532 nm, the working range of the instrument was established between 0.2 μmol L-1 and 8.0 μmol L-1 using 1,1,3,3 as a calibration standard. -tetraethoxypropane (TEP), the LDI, LDM and LCM were 0.01, 0.04 and 0.09 mg kg-1 respectively, expressed in Malondialdehyde (MDA) per kilogram of fresh sample, the working range of the method has as the lower limit to the LQM and as the upper limit to the value of 2.50 mg kg-1 and the coefficients of variation for the repeatability and intermediate precision tests were less than 11% and 16%.
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