He Advanced Optical Microscopy Applied to the Study of the Retina: Analysis of Its Potential Use as a Diagnostic Tool for Ocular Pathology: Optical Microscopy in retina

Abstract

The retina is potentially affected both directly by the passage of time and indirectly by the way in which this time has elapsed and by the extent to which it has been protected from or exposed to harmful factors. Recently, techniques based on retinal autofluorescence, such as confocal laser scanning microscopy (CLSM) and fluorescence lifetime imaging microscopy (FLIM), have found broad applications in ophthalmology, primarily because they are highly sensitive and non-invasive.

FLIM is an emerging imaging modality for measuring the fluorescence lifetimes of retinal fluorophores, including aromatic amino acids, chromophores, and certain pigments. The present project aims to investigate the retina using modern microscopy techniques (CLSM and FLIM), with the objective of identifying new diagnostic indicators.